Haha, thanks! I have a few years of experience with home mycology (agar work/sterile technique) and I also keep lots of houseplants, so I’m thinking some of these skills might transfer when I do get into it. A reason why I’m interested in this is because some of the work I’ve read about fungi-root interactions was performed on fungi grown on root tissue cultures - which makes sense - but I don’t really know how one would practically achieve that. So I’m very curious about it…
Gotcha. While never having cultured fungus (on purpose) plants definitely pose unique challenges. Such as hormone application. I also think, though I don’t have extensive knowledge and fungal media composition, that the media for plants can need quite a lot more sugar which makes bacteria and fungi even more a problem.
Really cool you’ve already done it at home though. Did you do pressure cooker method
Oh, I haven’t worked with hormones before. That would be interesting.
Did you do pressure cooker method
Yeah, my pressure cookers are basically my autoclaves. And I use a still-air box (30L transparent plastic tub with holes) for pouring plates and transfers.
https://en.wikipedia.org/wiki/Plant_tissue_culture?wprov=sfla1
:)
That’s intense
😱 Cool! This smells like the start of a new hobby…
Haha good luck! I do tissue culture for a living. You definitely can do it at home but you have a lot of work cut out for you
Haha, thanks! I have a few years of experience with home mycology (agar work/sterile technique) and I also keep lots of houseplants, so I’m thinking some of these skills might transfer when I do get into it. A reason why I’m interested in this is because some of the work I’ve read about fungi-root interactions was performed on fungi grown on root tissue cultures - which makes sense - but I don’t really know how one would practically achieve that. So I’m very curious about it…
Gotcha. While never having cultured fungus (on purpose) plants definitely pose unique challenges. Such as hormone application. I also think, though I don’t have extensive knowledge and fungal media composition, that the media for plants can need quite a lot more sugar which makes bacteria and fungi even more a problem.
Really cool you’ve already done it at home though. Did you do pressure cooker method
Oh, I haven’t worked with hormones before. That would be interesting.
Yeah, my pressure cookers are basically my autoclaves. And I use a still-air box (30L transparent plastic tub with holes) for pouring plates and transfers.
Oh yeah the hormones are cool you can make them do some weird stuff haha